Collect. Czech. Chem. Commun.
1979, 44, 2710-2721
https://doi.org/10.1135/cccc19792710
Amino acid sequence of basic acrosin inhibitor from bull seminal plasma
Bedřich Melouna and Dana Čechováb
a Institute of Organic Chemistry and Biochemistry
b Institute of Molecular Genetics, Czechoslovak Academy of Sciences, 166 10 Prague 6
Abstract
The molecule of the inhibitor contains 57 amino acid residues whose sequence is the following: Pyr-Gly-Ala-Gln-Val-Asp-Cys-Ala-Glu-Phe-Lys-Asp-Pro-Lys-Val-Tyr-Cys-Thr-Arg-His-Ser-Asp-Pro-Gln-Cys-Gly-Ser-Asn-Gly-Glu-Thr-Tyr-Gly-Asn-Lys-Cys-Ala-Phe-Cys-Lys-Ala-Val-Met-Lys-Ser-Gly-Gly-Lys-Ile-Asn-Leu-Lys-His-Arg-Gly-Cys-Lys. The N-terminal group of the inhibitor is pyrrolidone carboxylic acid. The sequential data were obtained by analyses of peptides isolated from tryptic and chymotryptic digests of the oxidized or carboxymethylated inhibitor. The molecular weight of the inhibitor is 6 200.
