Collection of Czechoslovak Chemical Communications - digital archive 

Abstract

Chromatographic properties of peptides derived from deamino-oxytocin, differing in the structure of the disulfide bridge region, were studied on a column with reversed phase. On the basis of chromatographic data the non-equivalence of the sulfur atoms in deamino-oxytocin was discussed. The analogues derived by oxidation or substitution of amino acids in the position 2 or 4 were also submitted to investigation. Rapid and selective oxidation of sulfide with periodate to sulfoxide, or the reduction of the latter with hydrogen bromide and acetone, was made use of for the determination of sulfide and sulfoxide in peptide containing sulfur-containing amino acid. The method was checked with free and protected peptides.