Collect. Czech. Chem. Commun. 1981, 46, 354-367
https://doi.org/10.1135/cccc19810354

The optical and acid-base properties of chromatographically pure Chromazurol S, Eriochromazurol B and Eriochromcyanine R

Naděžda Pollaková-Moukováa, Dagmar Gotzmannováb, Vlastimil Kubáňa and Lumír Sommera

a Department of Analytical Chemistry, Purkyně University, 611 37, Brno
b District Hospital and Health Center, Department of Clinical Biochemistry, 703 00 Ostrava-Zabreh

Abstract

The positons of the absorption maxima and the values of the molar absorption coefficients of all the acid-base forms and the dissociation constants of the individual acid-base transition of chromatographically pure Chromazurol S, Eriochromazurol B and Eriochromcyanine R were found by graphical and numerical interpretation of the absorbance curves measured in aqueous medium and mixed water-ethanol and water-DMF medium. These values were compared with the values given in the literature.