Collect. Czech. Chem. Commun.
1983, 48, 2249-2254
https://doi.org/10.1135/cccc19832249
7-Oxo-7H-benzimidazo[2,1-a]benz[d,e]isoquinoline-9-sulphonyl chloride as a new fluorescence reagent for identification and determination of aliphatic amines
Dagmar Tocksteinováa, Antonín Novákb, Hana Horákováa, Miloš Neprašb and Jaroslav Churáčeka
a Department of Analytical Chemistry, Institute of Chemical Technology, 532 10 Pardubice
b Research Institute of Organic Syntheses, 532 18 Pardubice-Rybitví
Abstract
Absorption and fluorescence properties have been studied of a new fluorescence reagent - 7-oxo-7H-benzimidazo[2,1-a]benz[d,e]isoquinoline-9-sulphonyl chloride and the corresponding sulphonamides derived from aliphatic amines. The fluorescence intensity is temperature-independent in the range 0-35 °C and depends linearly on concentration of the sulphonamides in the range 6 . 10-9 to 1 . 10-6 mol l-1. TLC separation of the sulphonamides is good, the yields of elution being 95%, which allows application of the procedure to determination of the amines.
