Collect. Czech. Chem. Commun.
1986, 51, 2050-2055
https://doi.org/10.1135/cccc19862050
Kinetics of the aflatoxin B1-sodium hypochlorite reaction and its application to the study of aflatoxin B1-bovine albumin binding
Guillermo A. Picó, María A. Picconi and Carlos A. Gatti
Instituto de Fisiología Experimental (IFISE), Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Rosario and Cátedra Fisicoquímica, Facultad de Ciencias Básicas, Universidad Nacional de Rosario, Suipacha 531, 2000 Rosario, Argentina
Abstract
The course of the aflatoxin B1-sodium hypochlorite reaction was followed by fluorescence emission at 450 nm. The formation of intermediate compounds with a higher fluorescence quantum yield than that of aflatoxin B1 was observed. This formation fitted a second order kinetics, and the measurement of the initial formation rate was used to calculate the concentration of the aflatoxin in solution. Bovine albumin exerted a protective effect on aflatoxin B1 against the sodium hypochlorite action, which was related to the hydrophobic nature of the aflatoxin B1-albumin binding. This effect was used to give a further insight of aflatoxin B1-albumin interaction.
