Collect. Czech. Chem. Commun. 1992, 57, 46-55
https://doi.org/10.1135/cccc19920046

Use of the Internal and External Standard Techniques in Determinations of Amino Acids by Ion Exchange Chromatography

Bohuslav Ritticha and Stanislav Standarab

a Institute of Systematic and Ecological Biology, 603 65 Brno
b Research Institute of Veterinary Medicine, 621 32 Brno

Abstract

Influence of internal standard technique on precision of amino acid determination was studied. The law of propagation of errors and the information theory was applied in this paper. From the results it follows that the internal standard technique did not inevitably improve the precision of the determination. In the case of multicomponent sample, the external standard technique improved the accuracy only if calibration curve was used.