Collect. Czech. Chem. Commun.
2006, 71, 804-819
https://doi.org/10.1135/cccc20060804
Synthesis of Oligoribonucleotides Containing Pyrimidine 2'-O-[(Hydroxyalkoxy)methyl]ribonucleosides
Georgii V. Bobkova, Kirill V. Brilliantova, Sergey N. Mikhailova,*, Jef Rozenskib, Arthur Van Aerschotb and Piet Herdewijnb
a Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov str. 32, Moscow 119991, Russia
b Rega Institute, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium
Abstract
A simple and efficient method for the preparation of pyrimidine 2'-O-[(2-hydroxyethoxy)methyl]ribonucleosides and 2'-O-[(3-hydroxypropoxy)methyl]ribonucleosides has been developed. These modified nucleosides were incorporated into oligoribonucleotides, which were shown to form stable RNA/RNA duplexes.
Keywords: Ribonucleosides; Oligonucleotides; 2'-O-Modified nucleosides; Antisense agents; RNA duplexes; Thermal denaturation; siRNA.
References: 29 live references.
