Collect. Czech. Chem. Commun.
2008, 73, 701-704
https://doi.org/10.1135/cccc20080701
Preparation of α-5-Aza-2'-deoxy-[6-3H]cytidine
Tomáš Elberta,* and Bohuslav Černýb
a Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10 Prague 6, Czech Republic
b Institute of Nuclear Medicine of the First Faculty of Medicine and General Teaching Hospital, Salmovská 3, 120 00 Prague 2, Czech Republic
Abstract
α-5-Aza-2'-deoxy cytidine was labeled by tritium on the C-6 of the heterocyclic triazine ring. The structure of the α-5-aza-2'-deoxy-[6-3H]cytidine and the position of the label was proved by 3H and 1H NMR. The specific activity was 0.71 TBq mmol-1 (19.2 Ci mmol-1) and radiochemical purity was >99%. The long term stability of the product during the storage at -21 and -72 °C was followed by radio-HPLC.
Keywords: α-5-Aza-2'-deoxy-cytidine; CESG; Tritium; 3H NMR.
References: 7 live references.
